A Study of Arbuscular Mycorrhizal Association in Three Species of Asterella (Aytoniaceae, Marchantiophyta) from Western Himalayan Region, India

Pratibha Kumari; Priti Giri; Prem Lal Uniyal

doi:10.30550/j.lil/2091

Authors

Pratibha Kumari Department of Botany, Daulat Ram College, University of Delhi, 110007, Delhi, India. https://orcid.org/0000-0002-1738-7526
Priti Giri Department of Botany, Faculty of Sciences, University of Delhi, 110007, Delhi, India. https://orcid.org/0000-0002-8332-9877
Prem Lal Uniyal Department of Botany, Faculty of Sciences, University of Delhi, 110007, Delhi, India. https://orcid.org/0000-0001-6412-1651

DOI:

https://doi.org/10.30550/j.lil/2091

Keywords:

AM, arbuscules, mycorrhizae, liverworts, Asterella, rhizosphere, Glomeromycotina

Abstract

Most plant species including bryophytes and pteridophytes, develop mutualistic connections with arbuscular mycorrhizal (AM) fungi that facilitate greater water and nutrient uptake in plants and provide enhanced resistance against various biotic and abiotic stresses. The present study explores the diversity of AM associated with the thalloid liverwort Asterella sp. Three species of Asterella (A. khasyana, A. blumeana, and A. wallichiana) were studied to determine their association with AM fungi. The fungal association present in both types of rhizoids (smooth-walled and tuberculate) shows a higher degree of colonization in the smooth walled rhizoids as compared to the tuberculate rhizoids, validating the differential role of absorption performed by both types of rhizoids. A. khasyana and A. blumeana show the presence of both septate and aseptate fungal hyphae in their rhizoids, confirming the association of more than one fungus. Apart from rhizoids, the storage and midrib regions of the thallus show intense fungal colonization. Various AM structures, such as arbuscules and vesicles, are also observed in both rhizoids and the thallus. Spores of nine fungal species belonging to two genera, Glomus and Gigaspora were isolated from the rhizosphere soil of A. Khasyana and A. wallichiana.

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References

Bischler, H. (1998). Systematics and evolution of the genera of the Marchantiales. Bryophyt. Biblioth 51: 1-201.

Bidartondo, M. I., Read, D. J., Trappe, J. M., Merckx, V., Ligrone, R. & Duckett, J. G. (2011). The dawn of symbiosis between plants and fungi. Biology Letters 7: 574-577. https://doi.org/10.1098/rsbl.2010.1203

Cao, J. G., Dai, X. L., Zou, H. M. & Wang, Q. X. (2014). Formation and development of rhizoids of the liverwort Marchantia polymorpha. The Journal of the Torrey Botanical Society 141 (2): 126-134. https://doi.org/10.3159/TORREY-D-13-00038.1

Cottet, A. C. & Messuti, M. I. (2022). New Record of Arbuscular Mycorrhiza in Asterella chilensis (Aytoniaceae, Marchantiophyta), Patagonia, Argentina. 2022. Boletín de la Sociedad Argentina de Botánica 57 (1).

Crandall-Stotler, B. & Stotler, R. E. (2008). Morphology and classification of the Marchantiophyta. In: Shaw A, Goffinet B. eds. Bryophyte biology. New York: Cambridge University Press, 1-54. https://doi.org/10.1017/CBO9780511754807.002

Duckett, J. G., Schmid, A. M. & Ligrone, R. (1998). Protonemal morphogenesis. In: Bates JW, Ashton NW, Duckett JG. eds. Bryology for the twenty-first century. Leeds, UK: British Bryological Society: 223-246. https://doi.org/10.1201/9781315138626

Field, K. J., Cameron, D. D., Leake, J. R., Tille, S., Bidartondo, M. I. & Beerling, D. J. (2012). Contrasting arbuscular mycorrhizal responses of vascular and non-vascular plants to a simulated Palaeozoic CO2 decline. Nature Communications 3 (1): 835.

Field, K. J., Rimington, W. R., Bidartondo, M. I., Allinson, K. E., Beerling, D. J., Cameron, D. D. et al. (2015). First evidence of mutualism between ancient plant lineages (Haplomitriopsida liverworts) and Mucoromycotina fungi and its response to simulated Palaeozoic changes in atmospheric CO2. New Phytologist 205 (2): 743-756.

Field, K. J., Rimington, W. R., Bidartondo, M. I., Allinson, K. E., Beerling, D. J., Cameron, D. D., Duckett, J. G., Leake, J. R. & Pressel, S. (2016). Functional analysis of liverworts in dual symbiosis with Glomeromycota and Mucoromycotina fungi under a simulated Palaeozoic CO2 decline. ISME Journal 10: 1514-1526. https://doi.org/10.1038/ismej.2015.204

Field, K. J. & Pressel, S. (2018). Unity in diversity: structural and functional insights into the ancient partnerships between plants and fungi. New Phytologist 220 (4): 996-1011. https://doi.org/10.1111/nph.15158

Gerdemann, J. W. & Nicolson, T. W. (1963). Spores of mycorrhizal Endogone species extracted from soil by wet-sieving and decanting method. Transactions of the British Mycological Society 46: 235-245.

Glime J. M. (2020). Bryophyte Ecology. Ebook: https://digitalcommons.mtu.edu/bryophyteecology/. Accessed 15 July 2020

Goffinet, B., Buck, W. R. & Shaw, A. J. (2008). Morphology, anatomy and classification of the Bryophyta. In: Shaw A, Goffinet B. eds. Bryophyte biology. New York: Cambridge University Press, 55-138. https://doi.org/10.1017/CBO9780511754807.002

Humphreys, C. P., Franks, P. J., Rees, M., Bidartondo, M. I., Leake, J. R. & Beerling, D. J. (2010). Mutualistic mycorrhiza- like symbiosis in the most ancient group of land plants. Nature Communications 1: 103. https://doi.org/10.1038/ ncomms1105

INVAM (2025). The International Collection of Vesicular Arbuscular Mycorrhizal Fungi. https://invam.ku.edu/

Jiang, Y., Wang, W., Xie, Q., Liu, N., Liu, L., Wang, D., Zhang, X., Yang, C., Chen, X. & Tang, D. (2017). Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi. Science 356: 1172-1175. https://doi.org/10.1126/science.aam9970

Kormanic, P. P. & McGraw, A. C., (1982). Quantification of vesicular-arbuscular mycorrhizas in plant roots. In Methods and Principles of Mycorrhizal Research (Ed. by N. C. Schenck), pp. 37-45. The American Phytopathological Society, St Paul, Minnesota.

Kowal, J., Pressel, S., Duckett, J. G., Bidartondo, M. I. & Field, K. J. (2018). From rhizoids to roots? Experimental evidence of mutualism between liverworts and ascomycete fungi. Annals of Botany 121: 221-227. https://doi.org/10.1093/aob/mcx126

Kumari, P., Chamola, B. P., Govindapyari, H., Gupta, R. & Uniyal, P. L. (2015). Studies on the diversity of soil fungi (AM) associated with bryophytes in Uttarakhand, North Western Himalaya, India. Annals of Forestry 23 (1):11-19.

Leake, J. R. & Read, D. J. (2016). Mycorrhizal symbiosis and pedogenesis throughout Earth’s history. In: N.C. Johnson & C. Gehring & J. Jansa (Eds). Mycorrhizal mediation of soil: fertility, structure and carbon storage (9-33). Amsterdam: Elsevier.

Long, D. G. (2006). Revision of the Genus Asterella P. Beauv. in Eurasia. Bryophytorum Bibliotheca 63: 1-299.

Ligrone, R., Carafa, A., Lumini, E., Bianciotto, V., Bonfante, P. & Duckett, J. G. (2007). Glomeromycotean associations in liverworts: a molecular, cellular and taxonomic analysis. American Journal of Botany 94: 1756-1777.

McConaha, M. (1941). Ventral structures effecting capillary in the Mrachantiales. American Journal of Botany 28: 301-306.

Nicolson, T. H. (1955). The mycotrophic habit in grasses (Ph.D. Thesis) University of Nottmgham.

Pressel, S., Bidartondo, M. I., Ligrone, R. & Duckett, J. G. (2010). Fungal symbioses in bryophytes: New insights in the twenty first century. Phytotaxa 9: 238-253.

https://doi.org/10.11646/phytotaxa.9.1.13

Parke, J. L. & Linderman, R. G. (1980). Association of vesicular-arbuscular mycorrhizal fungi with the moss Funaria hygrometrica. Canadian Journal of Botany 58: 1898-1904.

Redecker, D., Koder R. & Graham, L. E. (2000). Glomalean fungi from the Ordovician. Science 289: 1920-1921.

Rimington, W. R., Pressel, S., Duckett, J. G., Field, K. J. & Bidartondo, M. I. (2018) Ancient plants with ancient fungi: liverworts associate with early-diverging arbuscular mycorrhizal fungi. Proceedings of the Royal Society B. 285 (1888): 2018 2016.

Rimington, W. R., Duckett, J. G., Field, K. J., Bidartondo, M. I. & Pressel, S. (2020). The distribution and evolution of fungal symbioses in ancient lineages of land plants. Mycorrhiza 30 (1): 23-49.

Rousk, K., Degboe, J., Michelsen, A., Bradley, R. & Bellenger, J. P. (2017). Olybdenum and phosphorus limitation of moss-associated nitrogen fixation in boreal ecosystems. New Phytologist 214: 97-107. https://doi.org/10.1111/nph.14331

Schü?ler, A. (2000). Glomus claroideum forms and arbuscular mycorrhiza-like symbiosis with the hornwort Anthoceros punctatus. Mycorrhiza 10: 15-21. https://doi.org/10.1007/s005720050282

Schenck, N. C. & Perez, Y. (1990). A Manual for identification of vesicular arbuscular mycorrhizal fungi, INVAM, (3rd edn.) University of Florida, Florida.

Sharma, A. & Langer, A. (2017). Occurrence of mycorrhiza in a moss Encalypta vulgaris Hedw. Journal of Plant Development Sciences 9 (9): 881-883.

Turnau, K., Ronikier, M. & Unrug, J. (1999). Role of mycorrhizal links between plants in establishment of liverworts thalli in natural habitats. Acta Societatis Botanicorum Poloniae 68: 63-68. https://doi.org/10.5586/asbp.1999.011

van der Heijden, M. G., Martin, F. M., Selosse, M. A. & Sanders, I. R. (2015). Mycorrhizal ecology and evolution: the past, the present, and the future. New Phytologist 205: 1406-1423. https://doi.org/10.1111/nph.13288

van der Heijden, M. G., Dombrowski, N. & Schlaeppi, K. (2017). Continuum of root–fungal symbioses for plant nutrition. Proceedings of the National Academy of Sciences 114: 11574-11576. https://doi.org/10.1073/pnas.1716329114

Verma, M., Parihar, P. & Akhtar, O. (2022). Arbuscular Mycorrhizal Associations in Liverworts Plagiochasma Appendiculatum and Marchantia Papillata, an Evolutionary Amphibian Adaptation for Land Plant Colonization? Jundishapur Journal of Microbiology 15 (1): 7661-7679.

Xiao, B. & Bowker, M. A. (2020). Moss-biocrusts strongly decrease soil surface albedo, altering land-surface energy balance in a dryland ecosystem. Science of The Total Environment 741: 140425. https://doi.org/10.1016/j.scitotenv.2020.140425