Histomorfología e histoquímica del tubo digestivo en hembras de Leptodactylus latinasus (Anura: Leptodactylidae) durante el periodo reproductivo

Eliana Elizabeth Heredia Ojeda; Franco José  Pucci Alcaide; Adriana Azucena  Michel; Ana Pucci Alcaide

doi:10.30550/j.azl/2177

Authors

Eliana Elizabeth Heredia Ojeda Centro de Referencia para Lactobacilos (CERELA-CONICET) https://orcid.org/0009-0009-2103-0675
Franco José Pucci Alcaide Instituto de Morfología Animal, Dirección Zoología https://orcid.org/0000-0001-6509-7331
Adriana Azucena Michel Instituto de Morfología Animal, Dirección Zoología, Fundación Miguel Lillo https://orcid.org/0000-0002-5799-7003
Ana Pucci Alcaide Cátedra de Histología, Facultad de Ciencias Naturales e Inst. Miguel Lillo, Universidad Nacional de Tucumán https://orcid.org/0009-0000-2725-4391

DOI:

https://doi.org/10.30550/j.azl/2177

Keywords:

Histology, Digestion, Gastric glands, Reproduction

Abstract

The digestive tract is responsible for the digestion and absorption of
nutrients required for energy supply. The functional state of the organs
that compose it reflects a clear association between the animal’s habi-
tat and its physiology. The aim of this study is to perform a histological
analysis of the cellular and tissue structures of the esophagus, stomach,
small intestine, and large intestine, in order to contribute to a compre-
hensive understanding of the system and its influence on the repro-
ductive biology of the species. Fifteen female specimens of Leptodac-
tylus latinasus were dissected; the organs were removed, cleaned, and
processed using standard histological techniques. Tissue sections were
stained with Hematoxylin and Eosin, Mallory’s Trichrome, Alcian Blue
at pH 0.5 and 2.5, and Periodic Acid-Schiff (PAS). The histomorpholog-
ical analysis revealed features shared with other species; however, the
presence of gastric glands composed of (1) mucous neck cells, (2) chief
cells, and (3) parietal cells appear to be a trait not commonly observed
in most species. Furthermore, the histochemical analysis identified six
types of glands differentiated by the chemical nature of their contents.
This study demonstrates that female Leptodactylus latinasus specimens
exhibit histological characteristics along the digestive tract that enable
efficient digestion and nutrient assimilation. The high glandular activity
revealed by the histochemical analysis reflects an active feeding phase
during the reproductive period, ensuring energy reserves that support
successful egg-laying.

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