Análisis morfológico y filogenético del complejo Pluteus cervinus (Pluteaceae, Basidiomycota) revela la presencia de Pluteus hongoi y Pluteus primus en México

Daniel  Ulloa Olmos; María Olivia Rodríguez Alcántar; Darío Figueroa García; María del Pilar  Zamora Tavares; Ofelia Vargas Ponce; José Armando  Arias García; Jorge David López Pérez

doi:10.30550/j.lil/2333

Authors

Daniel Ulloa Olmos Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0009-0004-3517-1552
María Olivia Rodríguez Alcántar Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0002-4525-2952
Darío Figueroa García Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0001-9295-0959
María del Pilar Zamora Tavares Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0002-3202-7334
Ofelia Vargas Ponce Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0003-4139-503X
José Armando Arias García Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0002-7474-1878
Jorge David López Pérez Instituto de Botánica, Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Zapopan, Jalisco, México. https://orcid.org/0000-0003-0030-0495

DOI:

https://doi.org/10.30550/j.lil/2333

Keywords:

Biodiversity, Fungi, ITS, Phylogeny, Taxonomy

Abstract

Pluteus species within the Pluteus cervinus group can be difficult to delimit taxonomically even when molecular evidence is considered. They are widely distributed, grow in diverse habitats, and the lack of type material for P. cervinus complicates species taxonomic delimitation. The objective of this work is to phylogenetically study the Pluteus cervinus species complex occurring in Mexico, while also seeking describe and illustrate species of Pluteus not previously recorded for the country identified through phylogeny and corroborated by morphoanatomical analyses. Thirty-nine Mexican specimens identified as Pluteus cervinus, preserved at ENCB, FCME, ITCV, HUAA, MEXU, XAL, and IBUG, were studied, along with the type specimen of Pluteus pouzarianus (PRM). The macro- and micromorphological characteristics of the material were analyzed and described. Genetic analysis included 69 ITS region sequences from morphologically related taxa. The phylogenetic hypothesis was obtained using Maximum Likelihood and Bayesian Inference. Morphological variation and phylogenetic estimation allowed the identification of Pluteus petasatus, Pluteus hongoi, and Pluteus primus among the studied specimens, whereas Pluteus cervinus could not be confirmed. The occurrence of Pluteus hongoi and Pluteus primus in Mexico is validated for the first time. The evaluation of the phylogenetic relationships and morphological variation allowed a clear discrimination of the taxa recognized.

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