Effect of temperature stress on the growth, physiological and biochemical parameters, and enzymatic and non-enzymatic activities of two Nostoc strains from different habitats

Prabha  Tiwari

doi:10.30550/j.lil/2197

Autores

Prabha Tiwari Department of Botany, Goa University, Taleigao Plateau, Goa – 403 206, India https://orcid.org/0000-0001-9354-9112

DOI:

https://doi.org/10.30550/j.lil/2197

Palavras-chave:

Antioxidant enzyme, nitrogenase activity, oxidative damage, reactive oxygen species, temperature

Resumo

There is increasing evidence regarding the influence of elevated temperatures on the growth and productivity of photosynthetic organisms. In the current research, we compared physiological and biochemical responses of Nostoc spongiiforme Agardh ex Bornet et Flahault (1888) (freshwater) and Nostoc calcicola Brébisson ex Bornet & Flahault (1886) (marine water) by batch culture under various temperatures (25-45°C). A decrease in growth and photosynthetic pigment contents was observed with rising temperature in the N. spongiiforme, in contrast to N. calcicola. Furthermore, significantly higher levels of total peroxide and hydroxyl radicals were recorded at elevated temperatures, which in turn enhanced the accumulation of “malondialdehyde (MDA)” and carbonyl content, indicating greater oxidative damage in N. spongiiforme than N. calcicola. An increase in proline and “ascorbate (AsA)” content with rising temperature suggests that the cells of both Nostoc spp., in an attempt to mitigate the oxidative stress induced by temperature, showed higher proline and AsA content in N. calcicola than in N. spongiiforme. Likewise, an increase in the activity of superoxide dismutase (SOD) andcatalase (CAT) was also observed in N. calcicola in contrast to N. spongiiforme. The nitrogenease activity was also affected under different growth temperatures in both Nostoc spp. Hence, this study reveals that from the two Nostoc species studied, N. calcicole has the potential to thrive under changing climatic conditions. Further research will help in screening N. calcicola and the indentification of the genes that enable this cyanobacterium to thrive at higher temperatures, so they can be cultured in bulk and used for diverse biotechnological applications, even under extreme temperature conditions.

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